Abstract
A mecC (mecALGA251)-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA- and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacteriological Techniques / methods*
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DNA, Bacterial / genetics*
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Humans
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Methicillin-Resistant Staphylococcus aureus / genetics
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Methicillin-Resistant Staphylococcus aureus / isolation & purification*
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Molecular Diagnostic Techniques / methods*
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Multiplex Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Staphylococcal Infections / diagnosis
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Staphylococcal Infections / microbiology*
Substances
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Bacterial Proteins
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DNA, Bacterial