High-resolution typing by MLVF unveils extensive heterogeneity of European livestock-associated methicillin-resistant Staphylococcus aureus isolates with the sequence type 398

Int J Med Microbiol. 2013 Apr;303(3):124-7. doi: 10.1016/j.ijmm.2013.02.015. Epub 2013 Mar 19.

Abstract

Methicillin-resistant Staphylococcus aureus sequence type 398 (MRSA ST398) has emerged in livestock worldwide. In particular, areas in Europe with high densities of livestock farming are affected. Consequently, the incidence of human colonization and infection with ST398 is rapidly increasing. Distinguishing different ST398 isolates with standard typing tools is problematic. The objective of this study was to examine the discriminatory power of Multiple-Locus Variable number tandem repeat Fingerprinting (MLVF) on a highly diverse ST398 collection. Our data show that MLVF combined with spa-typing is an attractive approach for high-resolution typing of ST398 isolates and unveiling their relatedness.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier State / epidemiology
  • Carrier State / veterinary*
  • DNA Fingerprinting / methods
  • Europe / epidemiology
  • Genotype
  • Livestock / microbiology*
  • Methicillin-Resistant Staphylococcus aureus / classification*
  • Methicillin-Resistant Staphylococcus aureus / genetics*
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification
  • Minisatellite Repeats
  • Molecular Epidemiology / methods*
  • Molecular Typing / methods*
  • Staphylococcal Infections / epidemiology
  • Staphylococcal Infections / veterinary*
  • Staphylococcal Protein A / genetics

Substances

  • Staphylococcal Protein A