Contribution of molecular diagnosis to congenital toxoplasmosis

Laurence Delhaes; Hélène Yera; Sandrine Ache; Vassili Tsatsaris; Véronique Houfflin-Debarge

doi:10.1016/j.diagmicrobio.2013.02.008

Contribution of molecular diagnosis to congenital toxoplasmosis

Diagn Microbiol Infect Dis. 2013 Jun;76(2):244-7. doi: 10.1016/j.diagmicrobio.2013.02.008. Epub 2013 Mar 19.

Authors

Laurence Delhaes¹, Hélène Yera, Sandrine Ache, Vassili Tsatsaris, Véronique Houfflin-Debarge

Affiliation

¹ Mycology-Parasitology Laboratory, EA4547-BDEEP, CIIL, Faculté de Médecine, Univ Nord de France (UDSL), CHRU de Lille, France. [email protected]

PMID: 23518182
DOI: 10.1016/j.diagmicrobio.2013.02.008

Abstract

We evaluated the performance of three real-time polymerase chain reaction (PCR) assays on 73 samples from mothers and children with congenital toxoplasmosis. PCR assays had significantly higher sensitivity in prenatal period than in birth period when targeting the 529-bp repeat element (81.3% versus 36.0%) or the B1 gene (64.6% versus 20.0%).

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Child
DNA, Protozoan / isolation & purification
Female
Follow-Up Studies
Humans
Immunoglobulin G / blood
Pregnancy
Pregnancy Complications, Parasitic / diagnosis*
Prenatal Diagnosis / methods
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Toxoplasma / genetics
Toxoplasma / growth & development
Toxoplasma / isolation & purification
Toxoplasmosis, Congenital / diagnosis*

Substances

DNA, Protozoan
Immunoglobulin G