Conventional micropipette-based patch clamp techniques

Methods Mol Biol. 2013:998:91-107. doi: 10.1007/978-1-62703-351-0_7.

Abstract

The patch clamp technique revolutionized the study of ion channels and is considered the gold standard of measuring ion channel activity, from the academic laboratory to industrial-scale drug screening. This technique enables the study of ion channels, from single molecules up to the whole-cell ion channel population, and in their native environment. Whilst the study of single protein molecular behavior is the ultimate goal of biophysicists from all fields, this is a routine ability for ion channel specialists. This chapter is aimed at helping the beginner to design patch clamp experiments and to obtain the fundamental micropipette configurations with mammalian cells: cell-attached patch, whole cell, inside-out patch, and outside-out patch.

MeSH terms

  • Electric Conductivity
  • HEK293 Cells
  • Humans
  • Ion Channel Gating
  • Ion Channels / metabolism
  • Ligands
  • Microtechnology / methods*
  • Patch-Clamp Techniques / methods*

Substances

  • Ion Channels
  • Ligands