Seven years after the previous National Vaccines Plan (PNV) 2005-2007 the new PNPV 2012-2014 was ratified with the approval of both National and Regional Authorities in February 22, 2012. The implementation state of the new Plan at 31/12/2012 was evaluated in all Italian Regions. The regional situations of the new strategies introduced by the Plan regarding Human Papillomavirus, pneumococcal, meningococcal and varicella vaccines are described. This analysis indicates that in the field of immune prevention a better interregional coordination is needed, to achieve an efficient Italian vaccination strategy.