Highly efficient förster resonance energy transfer in a fast, serum-compatible immunoassay

Thomas Kreisig; Ralf Hoffmann; Thole Zuchner

doi:10.1002/cbic.201300073

Highly efficient förster resonance energy transfer in a fast, serum-compatible immunoassay

Chembiochem. 2013 Apr 15;14(6):699-702. doi: 10.1002/cbic.201300073. Epub 2013 Mar 26.

Authors

Thomas Kreisig¹, Ralf Hoffmann, Thole Zuchner

Affiliation

¹ Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Center for Biotechnology and Biomedicine, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.

PMID: 23532940
DOI: 10.1002/cbic.201300073

Abstract

Highly efficient FRET leads to important enhancements for homogeneous immunoassays. By using the novel phosphorescent dye EuLH and BHQ-10 as a donor-acceptor pair, the FRET efficiency increases to >99.5 %, leading to significantly improved signal-to-background ratio, precision and linear range. The phosphorescence detection enabled full compatibility to serum samples for this fast-responding immunoassay.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Fluorescence Resonance Energy Transfer / economics
Fluorescence Resonance Energy Transfer / methods*
Immunoassay / economics
Immunoassay / methods
Lanthanoid Series Elements / chemistry*
Luminescent Agents / chemistry*
Models, Molecular
Peptides / chemistry
Serum / chemistry*
Swine

Substances

Lanthanoid Series Elements
Luminescent Agents
Peptides