Abstract
Cysteine-cysteine chemokine receptor type 5 (CCR5) is an important co-receptor for human immunodeficiency virus (HIV) infection and CCR5 neutralizing agents have proven efficient in patients suffering from HIV infection. Here, we expressed and purified various CCR5 vaccines named rCCR5, PADRE-rCCR5, GST-C1 and GST-C2 composed of different epitopes of CCR5. Results showed that vaccines containing multiple epitopes (rCCR5 and PADRE-rCCR5) induced stronger immune responses than single-epitope ones (GST-C1 and GST-C2). In addition, the elicited antibodies can specifically bind CCR5(+) U937 but not CCR5(-) Wish cells. These results demonstrate that the CCR5 vaccines are useful for further research, especially for the in vitro preclinical evaluation of their potential as biological CCR5 neutralizing agents.
Copyright © 2013 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AIDS Vaccines / chemistry
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AIDS Vaccines / genetics*
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AIDS Vaccines / immunology
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AIDS Vaccines / therapeutic use*
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Amino Acid Sequence
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Animals
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Antibody Formation
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BALB 3T3 Cells
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Base Sequence
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Cell Line
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Cloning, Molecular
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Epitopes / chemistry
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Epitopes / genetics
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Epitopes / immunology
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Epitopes / therapeutic use
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Escherichia coli / genetics
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HIV Infections / immunology
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HIV Infections / prevention & control*
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HIV-1 / immunology*
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Humans
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Mice
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Molecular Sequence Data
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Plasmids / genetics
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Receptors, CCR5 / chemistry
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Receptors, CCR5 / genetics*
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Receptors, CCR5 / immunology
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Receptors, CCR5 / therapeutic use*
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Recombinant Proteins / therapeutic use
Substances
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AIDS Vaccines
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Epitopes
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Receptors, CCR5
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Recombinant Proteins