Abstract
Previous studies have investigated the activity and protein levels of BACE1, the β-secretase, in the brain and cerebrospinal fluid (CSF) of Alzheimer's disease (AD) patients, however, results remain contradictory. We present here a highly specific and sensitive BACE1 ELISA, which allows measuring accurately BACE1 levels in human samples. We find that BACE1 levels in CSF of AD patients and other neurological disorder (OND) patients are slightly increased when compared to those of a non-neurological disorder control group (NND). BACE1 levels in CSF were well correlated with total-tau and hyperphosphorylated tau levels in the CSF, suggesting that the recorded alterations in BACE1 levels correlate with cell death and neurodegeneration.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Alzheimer Disease / cerebrospinal fluid*
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Alzheimer Disease / diagnosis*
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Amyloid Precursor Protein Secretases / biosynthesis
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Amyloid Precursor Protein Secretases / cerebrospinal fluid*
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Amyloid Precursor Protein Secretases / chemistry
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Animals
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Aspartic Acid Endopeptidases / biosynthesis
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Aspartic Acid Endopeptidases / cerebrospinal fluid*
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Aspartic Acid Endopeptidases / chemistry
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Biomarkers / cerebrospinal fluid
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Female
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HEK293 Cells
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Humans
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Male
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Mice
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Mice, Knockout
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Nervous System Diseases / cerebrospinal fluid
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Nervous System Diseases / diagnosis
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Phosphorylation / physiology
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Protein Structure, Secondary
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Single-Blind Method
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tau Proteins / cerebrospinal fluid*
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tau Proteins / chemistry
Substances
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Biomarkers
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tau Proteins
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Amyloid Precursor Protein Secretases
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Aspartic Acid Endopeptidases
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BACE1 protein, human