WKYMVm-induced cross-talk between FPR2 and HGF receptor in human prostate epithelial cell line PNT1A

Fabio Cattaneo; Melania Parisi; Rosario Ammendola

doi:10.1016/j.febslet.2013.03.036

WKYMVm-induced cross-talk between FPR2 and HGF receptor in human prostate epithelial cell line PNT1A

FEBS Lett. 2013 May 21;587(10):1536-42. doi: 10.1016/j.febslet.2013.03.036. Epub 2013 Apr 11.

Authors

Fabio Cattaneo¹, Melania Parisi, Rosario Ammendola

Affiliation

¹ Department of Biochemistry and Medical Biotechnology, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.

PMID: 23583448
DOI: 10.1016/j.febslet.2013.03.036

Abstract

Cross-communication between GPCRs and TKRs represents a mechanism to amplify the information exchange throughout the cell. We show that WKYMVm, an FPR2 agonist, induces the phosphorylation of Y1313/Y1349/Y1356 residues of c-Met and triggers some of the molecular responses elicited by c-Met/HGF binding, such as STAT3, PLC-γ1/PKCα and PI3K/Akt pathways. The critical role of NADPH oxidase-dependent superoxide generation in this cross-talk mechanism is supported by the finding that blockade of NADPH oxidase function prevents c-Met trans-phosphorylation and the downstream signalling cascade. These results highlight the function of FPR2 to activate a interconnected signalling network and suggest novel possibilities for therapeutic interventions.

MeSH terms

Catalytic Domain / genetics
Cell Line
Epithelial Cells / cytology
Epithelial Cells / drug effects
Epithelial Cells / metabolism
Humans
Male
Oligopeptides / pharmacology*
Phosphorylation / drug effects
Prostate / cytology
Prostate / drug effects
Prostate / metabolism*
Proto-Oncogene Proteins c-met / antagonists & inhibitors
Proto-Oncogene Proteins c-met / chemistry
Proto-Oncogene Proteins c-met / genetics
Proto-Oncogene Proteins c-met / metabolism*
RNA, Small Interfering / pharmacology
Receptor Cross-Talk / drug effects*
Receptors, Formyl Peptide / agonists
Receptors, Formyl Peptide / antagonists & inhibitors
Receptors, Formyl Peptide / genetics
Receptors, Formyl Peptide / metabolism*
Receptors, Lipoxin / agonists
Receptors, Lipoxin / antagonists & inhibitors
Receptors, Lipoxin / genetics
Receptors, Lipoxin / metabolism*
Signal Transduction / drug effects
Signal Transduction / genetics
Tyrosine / genetics
Tyrosine / metabolism

Substances

FPR2 protein, human
Oligopeptides
RNA, Small Interfering
Receptors, Formyl Peptide
Receptors, Lipoxin
Trp-Lys-Tyr-Met-Val-Met
Tyrosine
Proto-Oncogene Proteins c-met