Immunohistochemical demonstration of cyclooxygenase-2 in glomus tumors

J Bone Joint Surg Am. 2013 Apr 17;95(8):725-8. doi: 10.2106/JBJS.L.00341.

Abstract

Background: Glomus tumors are benign hamartomas that account for 1% to 5% of all soft-tissue tumors of the hand. Painful spasms radiating from the lesion are typical clinical signs. As the pain production mechanism is unclear, we evaluated S100 protein, substance P, and cyclooxygenase-2 expression by immunohistochemistry in glomus tumor samples.

Methods: Eight solitary glomus tumors were surgically excised and confirmed histologically by an experienced pathologist. Paraffin-embedded tissues were prepared for immunohistochemistry. The sections were stained with separate polyclonal antibodies for S100, substance P, and cyclooxygenase-2. In three of the tumors, we measured the prostaglandin E2 concentrations.

Results: All samples were positive for S100 protein and cyclooxygenase-2.Substance P was found in five of the eight samples. High prostaglandin-E2 concentrations were seen in all three samples tested.

Conclusions: Cyclooxygenase-2-immunoreactive cells are present in solitary glomus tumors. Since cyclooxygenase-2 produces prostaglandin E2, which is thought to be a strong vasodilator, the pain could be caused by vasodilation in the glomus tumor, with increased intracapsular pressure.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Cohort Studies
  • Cyclooxygenase 2 / metabolism*
  • Female
  • Glomus Tumor / metabolism*
  • Glomus Tumor / pathology
  • Glomus Tumor / surgery
  • Hand*
  • Humans
  • Male
  • Middle Aged
  • Pain / etiology
  • Pain / metabolism*
  • Pain / pathology
  • S100 Proteins / metabolism
  • Soft Tissue Neoplasms / metabolism*
  • Soft Tissue Neoplasms / pathology
  • Soft Tissue Neoplasms / surgery
  • Substance P / metabolism

Substances

  • S100 Proteins
  • Substance P
  • Cyclooxygenase 2