Enhancement of the immune responses to foot-and-mouth disease vaccination in mice by oral administration of a novel polysaccharide from the roots of Radix Cyathulae officinalis Kuan (RC)

Haibo Feng; Xiaogang Du; Jing Tang; Xiaohan Cao; Xingfa Han; Zhiyu Chen; Yanger Chen; Xianyin Zeng

doi:10.1016/j.cellimm.2013.02.004

Enhancement of the immune responses to foot-and-mouth disease vaccination in mice by oral administration of a novel polysaccharide from the roots of Radix Cyathulae officinalis Kuan (RC)

Cell Immunol. 2013 Feb;281(2):111-21. doi: 10.1016/j.cellimm.2013.02.004. Epub 2013 Mar 13.

Authors

Haibo Feng¹, Xiaogang Du, Jing Tang, Xiaohan Cao, Xingfa Han, Zhiyu Chen, Yanger Chen, Xianyin Zeng

Affiliation

¹ Isotope Research Laboratory, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.

PMID: 23603047
DOI: 10.1016/j.cellimm.2013.02.004

Abstract

Foot-and-mouth disease (FMD) is a kind of the important animal infectious disease caused by the foot-and-mouth disease virus (FMDV). Thus, the aim of this study was to determine the effects of the polysaccharide from the Radix Cyathulae officinalis Kuan (RCPS) for its adjuvant potential on the FMDV-specific cellular and humoral immune responses in mice. In this study, our findings shows that oral administration of RCPS significantly enhanced the phagocytic capacity of peritoneal macrophage, splenocyte proliferation, the activity of natural killer (NK) cells and cytotoxic T lymphocytes (CTL) and IgG, IgG1, IgG2a, and IgG2b antibody titers. Furthermore, RCPS promoted the level of IL-2, IFN-γ and IL-4 in CD4(+)T cells and level of IFN-γ in CD8(+)T cells. In addition, RCPS enhanced the expression of CD40(+), CD80(+) and CD86(+) on DCs. Importantly, RCPS could up-regulated the mRNA level of MHC I, MHC II, TLR-2, TLR-4. Interestingly, RCPS down-regulated the frequency of CD4(+)CD25(+)Foxp3(+)Treg cells. Taken together, these results demonstrate that RCPS can enhance both cellular and humoral immune responses via up-regulating DCs maturation through TLR2, TLR4 signaling pathway, and suppressing Treg frequency.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic / administration & dosage
Administration, Oral
Amaranthaceae / chemistry*
Animals
Cytokines / immunology
Cytokines / metabolism
Dendritic Cells / drug effects
Dendritic Cells / immunology
Dendritic Cells / metabolism
Female
Foot-and-Mouth Disease / immunology*
Foot-and-Mouth Disease / prevention & control
Foot-and-Mouth Disease / virology
Foot-and-Mouth Disease Virus / immunology*
Humans
Immunoglobulin G / immunology
Immunoglobulin G / metabolism
K562 Cells
Killer Cells, Natural / drug effects
Killer Cells, Natural / immunology
Macrophages, Peritoneal / drug effects
Macrophages, Peritoneal / immunology
Major Histocompatibility Complex / genetics
Major Histocompatibility Complex / immunology
Mice
Mice, Inbred ICR
Phagocytosis / immunology
Plant Roots / chemistry*
Polysaccharides / administration & dosage
Polysaccharides / immunology*
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocytes / drug effects
T-Lymphocytes / immunology
T-Lymphocytes / metabolism
T-Lymphocytes, Regulatory / drug effects
T-Lymphocytes, Regulatory / immunology
T-Lymphocytes, Regulatory / metabolism
Toll-Like Receptor 2 / genetics
Toll-Like Receptor 2 / immunology
Vaccination

Substances

Adjuvants, Immunologic
Cytokines
Immunoglobulin G
Polysaccharides
TLR2 protein, human
Toll-Like Receptor 2