Abstract
We report 1) the anchoring of preformed fluorescent DNA nanodevices (NDs) and 2) the in situ self-assembly of fluorescent DNA NDs on target living cell surfaces. Three types of aptamer-tethered DNA NDs were built and anchored on target cell surfaces by specific target-aptamer association. The in situ nanodevice self-assembly was further demonstrated on the surfaces of target living cells in cell mixtures. These DNA NDs exhibited fluorescence emission and underwent fluorescence energy transfer on living cell surfaces.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aptamers, Nucleotide / chemistry*
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Aptamers, Nucleotide / metabolism
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Cell Line
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Cell Membrane / metabolism*
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DNA / chemistry*
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DNA / metabolism
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Electrophoresis, Agar Gel
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Flow Cytometry
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Fluorescence Resonance Energy Transfer
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Fluorescent Dyes / chemistry
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Microscopy, Confocal
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Molecular Probes / chemistry
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Nanostructures / chemistry*
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Nanotechnology / instrumentation
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Nanotechnology / methods*
Substances
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Aptamers, Nucleotide
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Fluorescent Dyes
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Molecular Probes
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DNA