Rolling circle amplification-based DNA origami nanostructrures for intracellular delivery of immunostimulatory drugs

Xiangyuan Ouyang; Jiang Li; Huajie Liu; Bin Zhao; Juan Yan; Yinzhou Ma; Shoujun Xiao; Shiping Song; Qing Huang; Jie Chao; Chunhai Fan

doi:10.1002/smll.201300458

Rolling circle amplification-based DNA origami nanostructrures for intracellular delivery of immunostimulatory drugs

Small. 2013 Sep 23;9(18):3082-7. doi: 10.1002/smll.201300458. Epub 2013 Apr 24.

Authors

Xiangyuan Ouyang¹, Jiang Li, Huajie Liu, Bin Zhao, Juan Yan, Yinzhou Ma, Shoujun Xiao, Shiping Song, Qing Huang, Jie Chao, Chunhai Fan

Affiliation

¹ Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Center, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.

PMID: 23613456
DOI: 10.1002/smll.201300458

Abstract

Several single-stranded scaffold DNA, obtained from rolling circle amplification (RCA), are folded by different staples to form DNA nanoribbons. These DNA nanoribbons are rigid, simple to design, and cost-effective drug carriers, which are readily internalized by mammalian cells and show enhanced immunostimulatory activity.

Keywords: DNA nanostructures; DNA origami; cellular uptake; drug delivery; rolling circle amplification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic / administration & dosage*
Animals
DNA / chemistry*
Drug Carriers / chemistry*
Humans
Nanostructures / chemistry*
Nanotubes, Carbon / chemistry*

Substances

Adjuvants, Immunologic
Drug Carriers
Nanotubes, Carbon
DNA