Fourier transform infrared microspectroscopy reveals unique phenotypes for human embryonic and induced pluripotent stem cell lines and their progeny

J Biophotonics. 2014 Oct;7(10):767-81. doi: 10.1002/jbio.201200217. Epub 2013 Apr 25.

Abstract

Fourier transform infrared (FTIR) microspectroscopy was employed to elucidate the macromolecular phenotype of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) and their differentiated progeny. Undifferentiated hESCs and hiPSC lines were found to be not clearly distinguishable from each other. However, although both hESC and hiPSC variants appeared to undergo similar changes during differentiation in terms of cell surface antigens, the derived cell types from all cell lines could be discriminated using FTIR spectroscopy. We foresee a possible future role for FTIR microspectroscopy as a powerful and objective investigative and quality control tool in regenerative medicine.

Keywords: Fourier transform infrared microspectroscopy; human embryonic stem cells; human induced pluripotent stem cells; regenerative medicine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / metabolism
  • Cell Culture Techniques
  • Cell Differentiation / physiology*
  • Cell Line
  • Discriminant Analysis
  • Embryonic Stem Cells / physiology*
  • Fibroblasts / physiology
  • Flow Cytometry
  • Humans
  • Induced Pluripotent Stem Cells / physiology*
  • Least-Squares Analysis
  • Mice
  • Multivariate Analysis
  • Principal Component Analysis
  • Spectroscopy, Fourier Transform Infrared / methods*

Substances

  • Antigens, Surface