Bone marrow processing for transplantation using Cobe Spectra cell separator

Transfus Apher Sci. 2013 Jun;48(3):359-63. doi: 10.1016/j.transci.2013.04.017. Epub 2013 Apr 28.

Abstract

Concentration of bone marrow aspirates is an important prerequisite prior to infusion of ABO incompatible allogeneic marrow and prior to cryopreservation and storage of autologous marrow. In this paper we present our experience in processing 15 harvested bone marrow for ABO incompatible allogeneic and autologous bone marrow (BM) transplantation using Cobe Spectra® cell separator. BM processing resulted in the median recovery of 91.5% CD34+ cells, erythrocyte depletion of 91% and volume reduction of 81%. BM processing using cell separator is safe and effective technique providing high rate of erythrocyte depletion and volume reduction, and acceptable recovery of the CD34+ cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ABO Blood-Group System
  • Adolescent
  • Anemia, Aplastic / therapy
  • Antigens, CD34 / metabolism
  • Blood Group Incompatibility
  • Bone Marrow / metabolism*
  • Bone Marrow / pathology
  • Bone Marrow Cells / cytology
  • Bone Marrow Transplantation / methods*
  • Cell Separation / instrumentation*
  • Cell Separation / methods*
  • Child
  • Child, Preschool
  • Erythrocytes / cytology
  • Female
  • Humans
  • Infant
  • Leukemia, Myeloid, Acute / therapy
  • Lymphohistiocytosis, Hemophagocytic / therapy
  • Lymphoma, Non-Hodgkin / therapy
  • Male
  • Myelodysplastic Syndromes / therapy
  • Neuroblastoma / therapy
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / therapy

Substances

  • ABO Blood-Group System
  • Antigens, CD34