A simple shotgun proteomics method for rapid bacterial identification

Dobryan M Tracz; Stuart J McCorrister; Patrick M Chong; David M Lee; Cindi R Corbett; Garrett R Westmacott

doi:10.1016/j.mimet.2013.04.008

A simple shotgun proteomics method for rapid bacterial identification

J Microbiol Methods. 2013 Jul;94(1):54-7. doi: 10.1016/j.mimet.2013.04.008. Epub 2013 Apr 28.

Authors

Dobryan M Tracz¹, Stuart J McCorrister, Patrick M Chong, David M Lee, Cindi R Corbett, Garrett R Westmacott

Affiliation

¹ National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.

PMID: 23631909
DOI: 10.1016/j.mimet.2013.04.008

Abstract

Bacterial pathogens were rapidly identified by shotgun proteomics using a novel, easy to implement database search strategy. Peptide sequence data from nano-LC-MS/MS was searched against a database represented by concatenated proteomes of completed genome sequences. Select bacterial species, including BSL-3 select agents, were used to demonstrate this method.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / chemistry*
Bacteria / classification*
Bacterial Proteins / analysis*
Bacteriological Techniques / methods*
Chromatography, Liquid / methods
Databases, Genetic
Proteome / analysis
Proteomics / methods*
Tandem Mass Spectrometry / methods

Substances

Bacterial Proteins
Proteome