Abstract
Proline accumulation is an important mechanism for osmotic regulation under salt stress. In this study, we evaluated proline accumulation profiles in roots, stems and leaves of Jerusalem artichoke (Helianthus tuberosus L.) plantlets under NaCl stress. We also examined HtP5CS, HtOAT and HtPDH enzyme activities and gene expression patterns of putative HtP5CS1, HtP5CS2, HtOAT, HtPDH1, and HtPDH2 genes. The objective of our study was to characterize the proline regulation mechanisms of Jerusalem artichoke, a moderately salt tolerant species, under NaCl stress. Jerusalem artichoke plantlets were observed to accumulate proline in roots, stems and leaves during salt stress. HtP5CS enzyme activities were increased under NaCl stress, while HtOAT and HtPDH activities generally repressed. Transcript levels of HtP5CS2 increased while transcript levels of HtOAT, HtPDH1 and HtPDH2 generally decreased in response to NaCl stress. Our results supports that for Jerusalem artichoke, proline synthesis under salt stress is mainly through the Glu pathway, and HtP5CS2 is predominant in this process while HtOAT plays a less important role. Both HtPDH genes may function in proline degradation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Computational Biology
-
Crops, Agricultural / enzymology
-
Crops, Agricultural / genetics
-
Crops, Agricultural / metabolism
-
Crops, Agricultural / physiology
-
Gene Expression Profiling
-
Genes, Plant / genetics
-
Helianthus / enzymology
-
Helianthus / genetics
-
Helianthus / metabolism*
-
Helianthus / physiology
-
Proline / biosynthesis*
-
Proline / metabolism*
-
Sodium Chloride / pharmacology*
-
Stress, Physiological / drug effects*
-
Stress, Physiological / genetics
Grants and funding
The research is supported by grants from National Natural Science Foundation of China (31201842; 31201692; 41171216), National Key Technology R&D Program of the Ministry of Science and Technology (2011BAD13B09), the State Key Laboratory of Crop Genetics and Germplasm Enhancement (ZW2010004), the Doctoral Program of Higher Education of China (20120097120015), Fundamental Research Funds for the Central Universities (KYZ201206), the National High Technology Research and Development Program (“863” Program, 2011AA100209), the Priority Academic Program Development of Jiangsu Higher Education Institutions (RAPD program, 809001), the Scientific Research Foundation of the State Human Resource Ministry and Scientific & Technological Innovation Foundation for Young Scientists of Nanjing Agricultural University (Y201058), Jiangsu Agricultural Science and Technology Independent Innovation Fund Project [No. CX(12)1005-1 and 3], the Ministry of Science and Technology of Jiangsu Province (No. BE2011368), One-Hundred-Talent Plan of Chinese Academy of Sciences CAS), the CAS/SAFEA International Partnership Program for Creative Research Teams, the Science & Technology Development Plan of Shandong Province (2010GSF10208), the Science & Technology Development Plan of Yantai City (2011016; 20102450), Yantai Double-hundred High-end Talent Plan (XY-003-02), and 135 Development Plan of YIC-CAS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.