Abstract
Hypoxia caused injury and metabolic dysfunction of astrocytes, as indicated by a time-dependent loss of lactate dehydrogenase (LDH) activity and ATP content. The combination of 3.5 mM fructose-1,6-bisphosphate (FBP) and 7.5 mM glucose (GLC) reduced the decrease of ATP and prevented the loss of LDH. These data indicate that the combination of GLC + FBP protects astrocytes from hypoxia. The results also suggest that the maintainance of ATP concentration is the mechanism by which FBP prevents hypoxic injury.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adenosine Triphosphate / metabolism*
-
Animals
-
Astrocytes / cytology
-
Astrocytes / drug effects
-
Astrocytes / metabolism*
-
Cell Hypoxia / drug effects*
-
Cells, Cultured
-
Cerebral Cortex / cytology
-
Cerebral Cortex / drug effects
-
Cerebral Cortex / metabolism*
-
Fructosediphosphates / pharmacology*
-
Hexosediphosphates / pharmacology*
-
Rats
-
Rats, Inbred Strains
Substances
-
Fructosediphosphates
-
Hexosediphosphates
-
Adenosine Triphosphate
-
fructose-1,6-diphosphate