Evaluation of the new CE-IVD marked BD MAX Cdiff Assay for the detection of toxigenic Clostridium difficile harboring the tcdB gene from clinical stool samples

Paul O Verhoeven; Anne Carricajo; Sylvie Pillet; Alain Ros; Nathalie Fonsale; Elisabeth Botelho-Nevers; Frédéric Lucht; Philippe Berthelot; Bruno Pozzetto; Florence Grattard

doi:10.1016/j.mimet.2013.04.009

Evaluation of the new CE-IVD marked BD MAX Cdiff Assay for the detection of toxigenic Clostridium difficile harboring the tcdB gene from clinical stool samples

J Microbiol Methods. 2013 Jul;94(1):58-60. doi: 10.1016/j.mimet.2013.04.009. Epub 2013 May 3.

Authors

Paul O Verhoeven¹, Anne Carricajo, Sylvie Pillet, Alain Ros, Nathalie Fonsale, Elisabeth Botelho-Nevers, Frédéric Lucht, Philippe Berthelot, Bruno Pozzetto, Florence Grattard

Affiliation

¹ GIMAP EA 3064-Groupe Immunité des Muqueuses et Agents Pathogènes, University of Lyon, 42023 Saint-Etienne, France.

PMID: 23643507
DOI: 10.1016/j.mimet.2013.04.009

Abstract

The evaluation of the fully automated BD MAX Cdiff assay on a panel of 100 stool samples characterized by the Xpert C. difficile assay reported a high concordance between the two molecular assays (kappa coefficient of 0.96), which makes this new assay suitable for routine detection of toxigenic Clostridium difficile.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Automation, Laboratory / methods
Bacterial Proteins / genetics*
Bacterial Toxins / genetics*
Bacteriological Techniques / methods*
Clostridioides difficile / genetics
Clostridioides difficile / isolation & purification*
Clostridium Infections / diagnosis*
Clostridium Infections / microbiology
Diarrhea / diagnosis*
Diarrhea / microbiology
Feces / microbiology*
Humans
Molecular Diagnostic Techniques / methods*

Substances

Bacterial Proteins
Bacterial Toxins
toxB protein, Clostridium difficile