Cloning and characterization of a novel human BRMS1 transcript variant in hepatocellular carcinoma cells

Cancer Lett. 2013 Sep 1;337(2):266-75. doi: 10.1016/j.canlet.2013.04.030. Epub 2013 Apr 30.

Abstract

Breast cancer metastasis suppressor 1 (BRMS1) is able to suppress tumor metastasis without affecting primary tumor growth in various cancers. Here, we report a novel transcript variant of human BRMS1, termed BRMS1.vh. BRMS1.vh is identical to the major BRMS1 variant (BRMS1.v1) except for missing base pairs 683-775, encoding a 215-amino acid protein lacking a functional nuclear localization sequence. Expression of BRMS1.vh in hepatocellular carcinoma (HCC) cells suppressed NF-κB signaling pathway, sensitized cells to apoptotic stimuli, leading to suppressed tumor growth. Taken together, our results suggest a potential role for BRMS1.vh in regulating cell apoptosis and tumor growth in HCC.

Keywords: Apoptosis; Breast cancer metastasis suppressor 1 (BRMS1); Hepatocellular carcinoma (HCC); Transcript variant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Apoptosis
  • Carcinoma, Hepatocellular / genetics
  • Carcinoma, Hepatocellular / metabolism*
  • Carcinoma, Hepatocellular / pathology
  • Cell Line, Tumor
  • Cell Proliferation
  • Cloning, Molecular*
  • Female
  • Genes, Reporter
  • Genetic Variation*
  • Humans
  • Liver Neoplasms / genetics
  • Liver Neoplasms / metabolism*
  • Liver Neoplasms / pathology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Molecular Sequence Data
  • NF-kappa B / metabolism
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / metabolism*
  • Repressor Proteins
  • Signal Transduction
  • Time Factors
  • Transcription, Genetic*
  • Transfection
  • Tumor Burden

Substances

  • BRMS1 protein, human
  • NF-kappa B
  • Neoplasm Proteins
  • Repressor Proteins