Effects of water-holding capability of the PVF sponge on the adhesion and differentiation of rat bone marrow stem cell culture

J Biomed Mater Res A. 2014 Jan;102(1):247-53. doi: 10.1002/jbma.34695. Epub 2013 May 9.

Abstract

The aim of the study is to estimate the effects of the water-holding capability of the polyvinyl formal (PVF) sponges on osteogenic response in vitro experiments. The rat bone marrow stem cells (BMCs) were seeded and cultured for up to 4 weeks under static conditions in osteogenic media to evaluate the adhesion, proliferation, differentiation, and mineralization on the Dextran-coated PVF sponges with or without water-holding capability. The BMCs seeded onto the PVF sponges with water-holding capability showed more significant increases in DNA content, alkaline phosphatase (ALP) activity, osteocalcin content, and calcium deposition than those without water-holding capability. These results suggest that the Dextran-coated PVF sponges with high water-holding capability would have potential uses as both a new scaffold to bone tissue engineering and as a new biomaterial.

Keywords: bone marrow stem cells; coated polyvinyl formal sponge; high water‐holding capability; osteogenic ability.

MeSH terms

  • Animals
  • Antigens, Differentiation / biosynthesis
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism*
  • Cells, Cultured
  • Coated Materials, Biocompatible / chemistry*
  • Dextrans / chemistry*
  • Male
  • Osteogenesis*
  • Rats
  • Rats, Inbred F344
  • Stem Cells / cytology
  • Stem Cells / metabolism*
  • Water / chemistry*

Substances

  • Antigens, Differentiation
  • Coated Materials, Biocompatible
  • Dextrans
  • Water