Chryseobacterium yeoncheonense sp. nov., with ginsenoside converting activity isolated from soil of a ginseng field

Arch Microbiol. 2013 Jul;195(7):463-71. doi: 10.1007/s00203-013-0898-2. Epub 2013 May 12.

Abstract

A Gram-staining negative, aerobic, non-motile, non-flagellate, yellow-pigmented, rod-shaped bacterial strain, designated strain DCY67(T), was isolated from ginseng field in Republic of Korea. Strain DCY67(T) contained β-glucosidase activity which converts ginsenoside Rb1 to compound K. Optimum growth of DCY67(T) occurred at 30 °C and pH 6.0-6.5. Analysis of the 16S rRNA gene sequences revealed that strain DCY67(T) belonged to the family Flavobacteriaceae and was most closely related to Chryseobacterium ginsenosidimutans THG 15(T) (97.5 %). The genomic DNA G+C content was 36.1 mol%. The predominant quinones were MK-6 (90.9 %) and MK-7 (9.15 %). The major fatty acids were iso-C15:0, summed feature 3 (containing C16:1 ω7c and/or C16:1 ω6c) and iso-C17:0 3-OH. On the basis of these phenotypic, genotypic and chemotaxonomic studies, strain DCY67(T) represents a novel species of the genus Chryseobacterium, for which, name Chryseobacterium yeoncheonense sp. nov. proposed the type strain is DCY67(T) (=KCTC 32090(T) = JCM 18516(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Base Composition
  • Biotransformation
  • Chryseobacterium / classification
  • Chryseobacterium / cytology
  • Chryseobacterium / isolation & purification*
  • Chryseobacterium / physiology*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Genes, rRNA
  • Ginsenosides / metabolism*
  • Molecular Sequence Data
  • Panax / growth & development
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / analysis

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Fatty Acids
  • Ginsenosides
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 6
  • ginsenoside Rb1
  • ginsenoside M1

Associated data

  • GENBANK/JX141782