Detection of genetic translocations in lymphoma using fluorescence in situ hybridization

Methods Mol Biol. 2013:999:189-202. doi: 10.1007/978-1-62703-357-2_12.

Abstract

Certain lymphoma types are characterized by recurring genetic translocations. Detection of these translocations enables confirmation of a suspected diagnosis and provides a genetic marker which can be subsequently monitored and followed. Rapid and reliable identification of these molecular rearrangements is a key component in the workup of lymphoma. While conventional cytogenetics may be a useful tool in this regard, fluorescence in situ hybridization (FISH) offers additional advantages including the ability to use formalin-fixed tissues, no requirement for dividing cells, ability to score many cells, improved sensitivity, and faster turnaround time for results.

MeSH terms

  • Cytogenetics
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Lymphoma / diagnosis*
  • Lymphoma / genetics
  • Lymphoma / pathology
  • Translocation, Genetic / genetics*