Replication of white spot syndrome virus (WSSV) in the polychaete Dendronereis spp

Desrina; J A J Verreth; S B Prayitno; J H W M Rombout; J M Vlak; M C J Verdegem

doi:10.1016/j.jip.2013.05.002

Replication of white spot syndrome virus (WSSV) in the polychaete Dendronereis spp

J Invertebr Pathol. 2013 Sep;114(1):7-10. doi: 10.1016/j.jip.2013.05.002. Epub 2013 May 14.

Authors

Desrina¹, J A J Verreth, S B Prayitno, J H W M Rombout, J M Vlak, M C J Verdegem

Affiliation

¹ Aquaculture and Fisheries Group, Wageningen University, The Netherlands; Fisheries Department, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang, Indonesia. [email protected]

PMID: 23685030
DOI: 10.1016/j.jip.2013.05.002

Abstract

This study investigated whether WSSV replicates in naturally infected Dendronereis spp., a common polychaete (Nereididae) species in shrimp ponds in Indonesia. To detect WSSV replication, (i) immunohistochemistry (IHC) using a monoclonal antibody against WSSV VP28 protein and (ii) nested RT-PCR using specific primers set for the vp28 gene to detect WSSV-specific mRNA were applied. WSSV immunoreactive-nuclei were detected in the gut epithelium of the polychaete and WSSV mRNA was detected with nested RT-PCR. This, together with the IHC results, confirmed that WSSV could replicate in Dendronereis spp. This is the first report showing that WSSV replicated in a naturally infected non-crustacean host.

Keywords: Dendronereis spp.; Immunohistochemistry; RT-PCR; Replication; WSSV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Host-Pathogen Interactions
Polychaeta / virology*
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Virus Replication*
White spot syndrome virus 1 / genetics
White spot syndrome virus 1 / physiology*

Substances

RNA, Messenger