Post-dilution high convective transport improves microinflammation and endothelial dysfunction independently of the technique

Blood Purif. 2013;35(4):270-8. doi: 10.1159/000350611. Epub 2013 May 8.

Abstract

Background/aims: We examined the effects of different online hemodiafiltration techniques on microinflammation and endothelial damage/repair.

Methods: The study was designed as a prospective crossover study. Flow cytometry was used to measure CD14(+)CD16(+) monocytes, apoptotic endothelial microparticles (EMPs), and endothelial progenitor cells (EPCs).

Results: Patients treated with high-flux hemodialysis showed a marked chronic inflammatory state (HF-HD 11 ± 2) versus healthy subjects (HS 3.9 ± 2.3; p < 0.05). High convective transport, independent of the technique used, improves microinflammatory parameters (OL-HDF 7.3 ± 2.1 or MID 6.5 ± 3.4; p < 0.05) and the endothelial damage/repair balance compared to HF-HD (EPCs HF-HD 0.3 ± 0.2), with no differences found between the two modalities (EPCs OL-HDF 0.6 ± 0.1, MID 0.6 ± 0.2; p < 0.05).

Conclusion: An increase in convective transport improves the microinflammatory state and the endothelial damage/repair of these patients independently of the technique used.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Apoptosis*
  • Biomarkers / blood
  • Cell-Derived Microparticles / metabolism*
  • Cross-Sectional Studies
  • Endothelium, Vascular / injuries*
  • Endothelium, Vascular / metabolism*
  • Endothelium, Vascular / pathology
  • Female
  • Flow Cytometry
  • GPI-Linked Proteins / blood
  • Hemodiafiltration / adverse effects*
  • Humans
  • Inflammation / blood
  • Inflammation / etiology
  • Inflammation Mediators / blood*
  • Lipopolysaccharide Receptors / blood
  • Male
  • Middle Aged
  • Monocytes / metabolism
  • Monocytes / pathology
  • Prospective Studies
  • Receptors, IgG / blood

Substances

  • Biomarkers
  • FCGR3B protein, human
  • GPI-Linked Proteins
  • Inflammation Mediators
  • Lipopolysaccharide Receptors
  • Receptors, IgG