Identification of canine parvovirus with the Q370R point mutation in the VP2 gene from a giant panda (Ailuropoda melanoleuca)

Virol J. 2013 May 26:10:163. doi: 10.1186/1743-422X-10-163.

Abstract

Background: In this study, we sequenced and phylogenetic analyses of the VP2 genes from twelve canine parvovirus (CPV) strains obtained from eleven domestic dogs and a giant panda (Ailuropoda melanoleuca) in China. A novel canine parvovirus (CPV) was detected from the giant panda in China.

Results: Nucleotide and phylogenetic analysis of the capsid protein VP2 gene classified the CPV as a new CPV-2a type. Substitution of Gln for Arg at the conserved 370 residue in CPV presents an unusual variation in the new CPV-2a amino acid sequence of the giant panda and is further evidence for the continuing evolution of the virus.

Conclusions: These findings extend the knowledge on CPV molecular epidemiology of particular relevance to wild carnivores.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • China
  • Cluster Analysis
  • DNA, Viral / chemistry
  • DNA, Viral / genetics
  • Dogs
  • Evolution, Molecular
  • Molecular Sequence Data
  • Mutant Proteins / genetics
  • Mutation, Missense*
  • Parvovirus, Canine / classification
  • Parvovirus, Canine / genetics
  • Parvovirus, Canine / isolation & purification*
  • Phylogeny
  • Point Mutation
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Ursidae / virology*
  • Viral Structural Proteins / genetics*

Substances

  • DNA, Viral
  • Mutant Proteins
  • Viral Structural Proteins

Associated data

  • GENBANK/JX624761
  • GENBANK/JX624762
  • GENBANK/JX624763
  • GENBANK/JX624764
  • GENBANK/JX624765
  • GENBANK/JX624766
  • GENBANK/JX624767
  • GENBANK/JX624768
  • GENBANK/JX624769
  • GENBANK/JX624770
  • GENBANK/JX624771
  • GENBANK/JX624772