The mammalian germline as a pluripotency cycle

Development. 2013 Jun;140(12):2495-501. doi: 10.1242/dev.091603.

Abstract

Naive pluripotency refers to the capacity of single cells in regulative embryos to engender all somatic and germline cell types. Only germ cells - conventionally considered to be unipotent - can naturally re-acquire pluripotency, by cycling through fertilisation. Furthermore, primordial germ cells express, and appear to be functionally dependent upon, transcription factors that characterise the pluripotent state. We hypothesise that germ cells require pluripotency factors to control a de-restricted epigenome. Consequently, they harbour latent potential, as manifested in teratocarcinogenesis or direct conversion into pluripotent stem cells in vitro. Thus, we suggest that there exists an unbroken cycle of pluripotency, naive in the early epiblast and latent in the germline, that is sustained by a shared transcription factor network.

Keywords: DNA methylation; Embryonic germ cells; Embryonic stem cells; Germline; Pluripotency; Primordial germ cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst / cytology
  • Blastocyst / metabolism
  • Cell Differentiation
  • Cell Lineage
  • Cellular Reprogramming
  • DNA Methylation
  • Epigenesis, Genetic
  • Gametogenesis
  • Gene Expression Regulation, Developmental*
  • Germ Layers / cytology*
  • Germ Layers / metabolism
  • Male
  • Mammals
  • Mice
  • Octamer Transcription Factor-3 / genetics
  • Octamer Transcription Factor-3 / metabolism
  • Ovum / cytology*
  • Ovum / metabolism
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / metabolism
  • Spermatozoa / cytology
  • Spermatozoa / metabolism

Substances

  • Octamer Transcription Factor-3
  • Pou5f1 protein, mouse