[HPLC characteristic chromatographic profile of Sarcandra glabra]

Zhongguo Zhong Yao Za Zhi. 2013 Mar;38(6):856-60.
[Article in Chinese]

Abstract

Objective: To develop the characteristic chromatographic profile of Sarcandra glabra by HPLC for its quality control.

Method: The HPLC analysis was performed on an Agilent Zorbax Eclipse XDB-C18 column (4. 6 mm x 250 mm, 5 microm) with column temperature at 40 degree C. The mobile phase was consisted of water containing 0. 5% formic acid and acetonitrile to methanol (1:9) in gradient mode, and the detection wavelength was set at 344 nm.

Result: A common mode of the HPLC characteristic chromatographic profile has been establised. There were 20 common peaks , seven of which were identified, and 46 samples from different habitats were classified into five groups based on principal component cluster analysis.

Conclusion: The method was time-saving and can represent the chemical information and provide a scientific basis for quality control of S. glabra.

Publication types

  • English Abstract

MeSH terms

  • Chromatography, High Pressure Liquid
  • Cluster Analysis
  • Drugs, Chinese Herbal / chemistry
  • Drugs, Chinese Herbal / standards
  • Ferns / chemistry*
  • Organic Chemicals / analysis*
  • Organic Chemicals / chemistry
  • Organic Chemicals / isolation & purification
  • Quality Control

Substances

  • Drugs, Chinese Herbal
  • Organic Chemicals