Analysis of the transfer of circulating microRNA between cells mediated by gap junction

Methods Mol Biol. 2013:1024:87-96. doi: 10.1007/978-1-62703-453-1_7.

Abstract

A significant breakthrough in the field of research was the identification of microRNAs (miRNAs), which are small molecule, single-stranded nucleic acids. MiRNAs have diverse roles in cellular biology with the ability for translation in different areas of medicine. The size of miRNAs provides them for passage through gap junction. Recent studies have demonstrated the passage of miRNAs through gap junctional intercellular communication (GJIC) among cancer cells and between breast cancer cells and bone marrow stroma. The transfer of miRNAs has been implicated in the etiology of breast cancer dormancy. To this end, we have developed a miRNA reporter assay to assess the ability of miRNAs to be transferred between stem and breast cancer cells and target a specific recognition sequence.

MeSH terms

  • Biological Assay
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism*
  • Breast Neoplasms / chemistry*
  • Breast Neoplasms / pathology
  • Cell Communication
  • Cell Line, Tumor
  • Coculture Techniques
  • Exosomes / chemistry*
  • Exosomes / genetics
  • Female
  • Gap Junctions / metabolism*
  • Genes, Reporter
  • Humans
  • Luciferases / genetics
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • MicroRNAs / isolation & purification*
  • RNA Transport

Substances

  • MicroRNAs
  • Luciferases