Comparison of the sensitivities of three commercial assays for detection of the high risk HPV types 16, 18 and 45

J Virol Methods. 2013 Oct;193(1):147-50. doi: 10.1016/j.jviromet.2013.05.013. Epub 2013 May 30.

Abstract

The relative and analytical sensitivity of the APTIMA HPV test (AHPV, broad-spectrum, target amplification) and the PreTect HPV-Proofer (type-specific, target amplification) for the detection of HPV mRNA in various cell lines was compared. Equivalent relative sensitivity for the HPV 16-containing cell lines (2.5 cells/ml with both CaSki and SiHa) was observed for the mRNA assays--and similar sensitivities were observed for the detection of HPV 18 (HeLa) and 45 (MS751); ranging from 2.5 cells/ml (Proofer) to 25 cells/ml (APTIMA). In relation to analytical sensitivity, again, the mRNA assays showed similar sensitivities to each other, ranging from 0.1 to 1 cell per reaction for APTIMA and 0.1 to 10 cells per reaction for PreTect HPV-Proofer (depending on cell line). Both mRNA assays consistently achieved a higher analytical sensitivity than a DNA based comparator--the Hybrid Capture 2 High-Risk HPV DNA test (hc2). This study indicates that mRNA tests had high analytical sensitivity, higher than a well established DNA-test based when using cell lines as target. Implications for clinical application are discussed.

Keywords: APTIMA; HPV; PreTect HPV-Proofer; Sensitivity; hc2.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Female
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / virology
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • RNA, Viral / analysis
  • RNA, Viral / genetics
  • Sensitivity and Specificity
  • Virology / methods*

Substances

  • RNA, Messenger
  • RNA, Viral