Abstract
The major aim of this study is to elucidate the hypocholesterolemic mechanism exerted by rice protein (RP) in adult rats under cholesterol-enriched dietary condition. Compared with casein, the cholesterol levels in plasma and the liver were significantly reduced by RP, accompanying significant inhibition of cholesterol absorption. RP increased the activity and mRNA level of cholesterol 7α-hydroxylase, whereas acyl-CoA:cholesterol acyltransferase activity and gene expression were significantly depressed with consumption of RP. Neither the activity nor gene expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase of RP differed from that of casein. The gene expression of the peroxisome proliferator-activated receptor α and liver X receptor α were significantly activated by consumption of RP. RP did not modify the mRNA level of sterol regulatory element-binding protein-2 with respect to casein. These results suggest RP can induce a cholesterol-lowering effect through modifying cholesterol metabolism-related gene expression and enzyme activity in adult rats.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acyl Coenzyme A / genetics
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Acyl Coenzyme A / metabolism
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Animals
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Anticholesteremic Agents / pharmacology*
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Caseins / pharmacology
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Cholesterol / blood*
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Cholesterol 7-alpha-Hydroxylase / genetics
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Cholesterol 7-alpha-Hydroxylase / metabolism
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Cholesterol, Dietary / administration & dosage*
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Diet
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Dietary Proteins / pharmacology*
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Gene Expression / drug effects
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Hypercholesterolemia / genetics
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Hypercholesterolemia / metabolism
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Hypercholesterolemia / prevention & control
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Lipid Metabolism / drug effects*
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Lipid Metabolism / genetics
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Liver / drug effects
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Liver / metabolism
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Liver X Receptors
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Male
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Orphan Nuclear Receptors / genetics
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Orphan Nuclear Receptors / metabolism
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Oryza / chemistry*
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PPAR alpha / genetics
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PPAR alpha / metabolism
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Plant Proteins / pharmacology*
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RNA, Messenger / metabolism
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Rats
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Rats, Wistar
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Sterol O-Acyltransferase / genetics
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Sterol O-Acyltransferase / metabolism
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Sterol Regulatory Element Binding Proteins / genetics
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Sterol Regulatory Element Binding Proteins / metabolism
Substances
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Acyl Coenzyme A
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Anticholesteremic Agents
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Caseins
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Cholesterol, Dietary
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Dietary Proteins
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Liver X Receptors
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Nr1h3 protein, rat
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Orphan Nuclear Receptors
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PPAR alpha
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Plant Proteins
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RNA, Messenger
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Srebf2 protein, rat
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Sterol Regulatory Element Binding Proteins
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Cholesterol
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Cholesterol 7-alpha-Hydroxylase
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Sterol O-Acyltransferase