The application of molecular analyses for resolving taxonomic problems in the family Torticidae (Lepidoptera) is still uncommon. The majority of papers concern the assessment of population variability of economically important species; reports on the systematic positions ofNeotropical Tortricidae taxa are rare. The Neotropical genus Orthocomotis was classified initially as a member of the tribe Euliini. Then, based on genital morphology, it was moved to the tribe Polyorthini. A comparison of homologous 606 bp fragments of the COI mitochondrial gene revealed that Orthocomotis should be transfered back into the tribe Euliini. Based on an analysis ofphylogenetic relationships the studied genera of Euliini form a monophyletic cluster, clearly separated from tribe Polyorthini in which they were temporarily included. Moreover, in the current paper we describe two new species of the tribe Euliini: Galomecalpa lesta RAZOWSKI & PELZ, sp. n., Gauruncus ischyros RAZOWSKI & PELZ, sp. n.