Abstract
DNA hydroxylation catalyzed by Tet dioxygenases occurs abundantly in embryonic stem cells and neurons in mammals. However, its biological function in vivo is largely unknown. Here, we demonstrate that Tet1 plays an important role in regulating neural progenitor cell proliferation in adult mouse brain. Mice lacking Tet1 exhibit impaired hippocampal neurogenesis accompanied by poor learning and memory. In adult neural progenitor cells deficient in Tet1, a cohort of genes involved in progenitor proliferation were hypermethylated and downregulated. Our results indicate that Tet1 is positively involved in the epigenetic regulation of neural progenitor cell proliferation in the adult brain.
Copyright © 2013 Elsevier Inc. All rights reserved.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Aging / metabolism*
-
Animals
-
Cell Proliferation
-
Cognition*
-
DNA Methylation / genetics
-
DNA-Binding Proteins / deficiency
-
DNA-Binding Proteins / metabolism*
-
Dentate Gyrus / cytology
-
Gene Expression Regulation
-
Green Fluorescent Proteins / metabolism
-
Hippocampus / growth & development*
-
Hippocampus / metabolism*
-
Memory
-
Mice
-
Nestin / metabolism
-
Neurogenesis* / genetics
-
Neurons / cytology
-
Neurons / metabolism
-
Promoter Regions, Genetic / genetics
-
Proto-Oncogene Proteins / deficiency
-
Proto-Oncogene Proteins / metabolism*
-
Stem Cells / cytology
-
Stem Cells / metabolism
Substances
-
DNA-Binding Proteins
-
Nestin
-
Proto-Oncogene Proteins
-
TET1 protein, mouse
-
Green Fluorescent Proteins