Abstract
The synthesis and characterization of new biodegradable polymeric NPs loaded with bovine serum albumin marked with fluorescein isothiocyanate (FITC-BSA) is reported. The protein is encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) NPs by the double emulsion method with subsequent solvent evaporation. The NPs display a spherical shape with a narrow size distribution and no aggregation is observed after drying. Steady-state and time-resolved fluorescence measurements appear to be a sensitive method to investigate the protein environment on the nanometer-scale. Finally, FITC-BSA-loaded NPs are rapidly internalized in stem cells. Interestingly, 25% cells were slightly positive after 28 days.
Keywords:
PLGA; fluorescence; nanoparticles; stem cells.
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
MeSH terms
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Animals
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Biocompatible Materials / pharmacology*
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Biodegradation, Environmental
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Bone Marrow Cells / cytology
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Bone Marrow Cells / drug effects
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Bone Marrow Cells / metabolism
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Calorimetry, Differential Scanning
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Cattle
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Cells, Cultured
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Fluorescein-5-isothiocyanate / analogs & derivatives
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Fluorescein-5-isothiocyanate / metabolism
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Humans
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Immobilized Proteins / metabolism*
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Lactic Acid / chemistry*
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Mesenchymal Stem Cells / cytology*
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Mesenchymal Stem Cells / drug effects
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Mesenchymal Stem Cells / metabolism
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Nanoparticles / chemistry*
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Nanoparticles / ultrastructure
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Polyglycolic Acid / chemistry*
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Polylactic Acid-Polyglycolic Acid Copolymer
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Serum Albumin, Bovine / metabolism
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Spectrometry, Fluorescence
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Temperature
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Thermogravimetry
Substances
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Biocompatible Materials
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Immobilized Proteins
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fluorescein isothiocyanate bovine serum albumin
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Polylactic Acid-Polyglycolic Acid Copolymer
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Polyglycolic Acid
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Serum Albumin, Bovine
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Lactic Acid
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Fluorescein-5-isothiocyanate