Abstract
A cell-based phenotypic screen for inhibitors of biofilm formation in mycobacteria identified the small molecule TCA1, which has bactericidal activity against both drug-susceptible and -resistant Mycobacterium tuberculosis (Mtb) and sterilizes Mtb in vitro combined with rifampicin or isoniazid. In addition, TCA1 has bactericidal activity against nonreplicating Mtb in vitro and is efficacious in acute and chronic Mtb infection mouse models both alone and combined with rifampicin or isoniazid. Transcriptional analysis revealed that TCA1 down-regulates genes known to be involved in Mtb persistence. Genetic and affinity-based methods identified decaprenyl-phosphoryl-β-D-ribofuranose oxidoreductase DprE1 and MoeW, enzymes involved in cell wall and molybdenum cofactor biosynthesis, respectively, as targets responsible for the activity of TCA1. These in vitro and in vivo results indicate that this compound functions by a unique mechanism and suggest that TCA1 may lead to the development of a class of antituberculosis agents.
Keywords:
drug resistance; dual mechanism.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohol Oxidoreductases
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Amino Acid Sequence
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Animals
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Antitubercular Agents / administration & dosage
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Antitubercular Agents / chemistry
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Antitubercular Agents / pharmacology*
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Bacterial Proteins / antagonists & inhibitors
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Benzothiazoles / administration & dosage
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Benzothiazoles / chemistry
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Benzothiazoles / pharmacology*
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Biofilms / drug effects
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Biofilms / growth & development
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Carbohydrate Epimerases / antagonists & inhibitors
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Carbohydrate Epimerases / chemistry
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Carbohydrate Epimerases / genetics
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Drug Resistance, Bacterial
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Female
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Genes, Bacterial
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High-Throughput Screening Assays
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Isoniazid / administration & dosage
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Mice
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Mice, Inbred BALB C
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Mycobacterium tuberculosis / drug effects*
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Mycobacterium tuberculosis / enzymology
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Mycobacterium tuberculosis / genetics
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Oxidoreductases / antagonists & inhibitors
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Oxidoreductases / chemistry
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Oxidoreductases / genetics
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Rifampin / administration & dosage
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Thiophenes / administration & dosage
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Thiophenes / chemistry
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Thiophenes / pharmacology*
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Tuberculosis, Pulmonary / drug therapy*
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Tuberculosis, Pulmonary / microbiology
Substances
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Antitubercular Agents
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Bacterial Proteins
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Benzothiazoles
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TCA1 compound
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Thiophenes
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Oxidoreductases
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Alcohol Oxidoreductases
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DprE1 protein, Mycobacterium tuberculosis
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Carbohydrate Epimerases
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Isoniazid
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Rifampin