Probe ligation and real-time detection of KPC, OXA-48, VIM, IMP, and NDM carbapenemase genes

Diagn Microbiol Infect Dis. 2013 Aug;76(4):502-5. doi: 10.1016/j.diagmicrobio.2013.05.004. Epub 2013 Jun 18.

Abstract

The Check-MDR Carba test (Check-Points, Wageningen, Netherlands), which is based on specific molecular recognition of blaNDM, blaKPC, blaOXA-48, blaVIM, and blaIMP genes by DNA probe ligation and real-time PCR detection, was evaluated on 183 well-characterized Gram-negative rods. Representatives of the 5 gene families were accurately identified (specificities and sensitivities of 100%) within 4.5 hours. This test may be helpful to differentiate carbapenem resistance mediated by carbapenemases from those involving other mechanisms.

Keywords: Carbapenem resistance; Detection; Real-time PCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / classification
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Biological Assay / standards*
  • Carbapenems / pharmacology
  • DNA Primers / genetics
  • DNA Probes / genetics
  • Gene Expression
  • Gram-Negative Bacteria / drug effects
  • Gram-Negative Bacteria / enzymology
  • Gram-Negative Bacteria / genetics*
  • Gram-Negative Bacteria / isolation & purification
  • Humans
  • Isoenzymes / classification
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Microbial Sensitivity Tests
  • Real-Time Polymerase Chain Reaction / standards*
  • Sensitivity and Specificity
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / classification
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carbapenems
  • DNA Primers
  • DNA Probes
  • Isoenzymes
  • beta-Lactamases
  • carbapenemase