Sequential addition of reprogramming factors improves efficiency

Nat Cell Biol. 2013 Jul;15(7):725-7. doi: 10.1038/ncb2800.

Abstract

Addition of a specific set of transcription factors reprograms somatic cell nuclei to a pluripotent state. Sequential addition of these factors, rather than the simultaneous exposure used in standard protocols, improves reprogramming efficiency. This sequential method favours a transition through a state with enhanced mesenchymal characteristics before driving an epithelial transformation on the way to the pluripotent state.

Publication types

  • News
  • Comment

MeSH terms

  • Animals
  • Cellular Reprogramming*
  • Epithelial-Mesenchymal Transition*
  • Induced Pluripotent Stem Cells / pathology*
  • Kruppel-Like Factor 4
  • Kruppel-Like Transcription Factors / metabolism*
  • Octamer Transcription Factor-3 / metabolism*
  • Proto-Oncogene Proteins c-myc / metabolism*
  • SOXB1 Transcription Factors / metabolism*

Substances

  • Kruppel-Like Factor 4
  • Kruppel-Like Transcription Factors
  • Octamer Transcription Factor-3
  • Pou5f1 protein, mouse
  • Proto-Oncogene Proteins c-myc
  • SOXB1 Transcription Factors
  • Sox2 protein, mouse