[The effects and properties of sodium nucleinate used as a pyrogen working standard. 7. Pyrogen removal by filtration and pyrogen destruction by steam sterilization]

Pharmazie. 1990 Mar;45(3):198-200.
[Article in German]

Abstract

Solutions containing bacterial lipopolysaccharides (P) and sodium nucleinate (NN) solutions filtered through cellulose asbestos filters became pyrogen free. But the filters, with respect to the pyrogenicity of these solutions, became earlier exhausted in case of NN than in case of P. A 12-min heating to 121 degrees C in the autoclave led to a significant lowering of pyrogenicity both in P- and in NN-solutions. After a 2-h heating in same conditions both P- and NN-solutions in the chosen ranges of concentration became pyrogen free.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Chinchilla
  • Drug Stability
  • Filtration
  • Hot Temperature
  • Nucleic Acids / analysis
  • Nucleic Acids / isolation & purification
  • Nucleic Acids / pharmacology*
  • Pyrogens / analysis
  • Pyrogens / isolation & purification
  • Pyrogens / pharmacology*
  • Rabbits
  • Reference Standards
  • Sterilization

Substances

  • Nucleic Acids
  • Pyrogens
  • sodium nucleinate