Abstract
We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anthraquinones / antagonists & inhibitors
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Anthraquinones / metabolism
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Anti-Bacterial Agents / biosynthesis*
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Gene Expression Regulation, Bacterial
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Genes, Bacterial*
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Peptide Nucleic Acids / genetics*
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RNA Interference*
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RNA, Antisense / genetics*
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Streptomyces coelicolor / genetics*
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Streptomyces coelicolor / growth & development
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Streptomyces coelicolor / metabolism
Substances
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Anthraquinones
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Anti-Bacterial Agents
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Peptide Nucleic Acids
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RNA, Antisense
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actinorhodin