Abstract
A recently developed class of models incorporating the cyton model of population generation structure into a conservation-based model of intracellular label dynamics is reviewed. Statistical aspects of the data collection process are quantified and incorporated into a parameter estimation scheme. This scheme is then applied to experimental data for PHA-stimulated CD4+T and CD8+T cells collected from two healthy donors. This novel mathematical and statistical framework is shown to form the basis for accurate, meaningful analysis of cellular behaviour for a population of cells labelled with the dye carboxyfluorescein succinimidyl ester and stimulated to divide.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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CD4-Positive T-Lymphocytes / drug effects
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CD4-Positive T-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / drug effects
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CD8-Positive T-Lymphocytes / immunology
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Cell Proliferation / drug effects
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Data Interpretation, Statistical
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Flow Cytometry / statistics & numerical data*
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Fluoresceins
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Fluorescent Dyes
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Humans
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Mathematical Concepts
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Mitogens / pharmacology
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Models, Immunological
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Models, Statistical
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Phytohemagglutinins / pharmacology
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Succinimides
Substances
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5-(6)-carboxyfluorescein diacetate succinimidyl ester
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Fluoresceins
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Fluorescent Dyes
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Mitogens
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Phytohemagglutinins
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Succinimides