Anti-leukaemic activity of a novel haploidentical-transplantation approach employing unmanipulated bone marrow followed by CD6-depleted peripheral blood stem cells in children with refractory/relapsed acute leukaemia

Br J Haematol. 2013 Sep;162(6):802-7. doi: 10.1111/bjh.12455. Epub 2013 Jul 6.

Abstract

The treatment outcome of children with refractory acute leukaemia or relapse post-stem cell transplantation is dismal. We report 10 children (non-remission n = 7) who underwent a new haploidentical transplant approach utilizing unmanipulated bone marrow followed by CD6-depleted peripheral blood stem cells. Nine patients had successful engraftment and no evidence of leukaemia. Acute and chronic graft-versus-host-disease was observed in five and three patients, respectively; two patients died of treatment-related toxicity. Seven patients relapsed after 7 (range 3-34) months, however two patients are alive at 6·5 and 7·0 years. This approach provides anti-leukaemic activity even in heavily pre-treated children but long-term disease control requires further intervention.

Keywords: CD6-depletion; acute refractory leukaemia; graft-versus-leukaemia-effect; haploidentical transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Antigens, CD / immunology*
  • Antigens, Differentiation, T-Lymphocyte / immunology*
  • Bone Marrow Transplantation / methods*
  • Child
  • Child, Preschool
  • Graft vs Host Disease / immunology
  • Haploidy
  • Hematopoietic Stem Cell Transplantation / methods*
  • Hematopoietic Stem Cells / immunology*
  • Humans
  • Infant
  • Leukemia / immunology
  • Leukemia / surgery*
  • Lymphocyte Depletion / methods
  • Neoplasm Recurrence, Local / surgery
  • Treatment Outcome

Substances

  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • CD6 antigen