Selecting criteria for improving results of an immunochromatographic assay for Mycobacterium tuberculosis complex identification from BacT/ALERT cultures

María José Munoz-Davila; Emilio Leiva Tápia; Ignacio Segura Díaz; María Aurora Cejudo García; Miguel José Martinez-Lirola

doi:10.1016/j.mimet.2013.06.022

Selecting criteria for improving results of an immunochromatographic assay for Mycobacterium tuberculosis complex identification from BacT/ALERT cultures

J Microbiol Methods. 2013 Sep;94(3):232-4. doi: 10.1016/j.mimet.2013.06.022. Epub 2013 Jul 3.

Authors

María José Munoz-Davila¹, Emilio Leiva Tápia, Ignacio Segura Díaz, María Aurora Cejudo García, Miguel José Martinez-Lirola

Affiliation

¹ Microbiology laboratory, Clinic Biotechnology Management Unit, Torrecardenas Hospital Complex, Almeria, Spain. [email protected]

PMID: 23830849
DOI: 10.1016/j.mimet.2013.06.022

Abstract

We describe a combined macro and microscopic criteria for rapid presumptive differentiation between Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM) evaluated by two independent observers. This strategy achieved rapid MTC identification in most cases (95.8% expert observer and 91.6% novice observer) with significant savings compared to more expensive and unnecessary tests.

Keywords: Criteria; Definitive identification; Presumptive differentiation; Saving.

MeSH terms

Bacterial Typing Techniques / methods*
Chromatography, Affinity / methods*
Humans
Microscopy, Fluorescence
Mycobacterium Infections / microbiology
Mycobacterium tuberculosis / classification
Mycobacterium tuberculosis / isolation & purification*
Nontuberculous Mycobacteria / classification
Nontuberculous Mycobacteria / isolation & purification*
Sensitivity and Specificity
Sputum / microbiology