Abstract
The long term in vivo biocompatibility is an essential feature for the design and development of sustained drug release carriers. In the recent intraocular drug delivery studies, hydrogels were suggested as sustained release carriers. The biocompatibility test for these hydrogels, however, was commonly performed only through in vitro cell culture examination, which is insufficient before the clinical applications. We compared three thermosensitive hydrogels that have been suggested as the carriers for drugs by their gel-solution phase-change properties. A new block terpolymer (PEOz-PCL-PEOz, ECE) and two commercial products (Matrigel® and Pluronic F127) were studied. The results demonstrated that the ocular media remained translucent for ECE and Pluronic F127 in the first 2 weeks, but cataract formation for Matrigel occurred in 2 weeks and for Pluronic F127 in 1 month, while turbid media was observed for both Matrigel and Pluronic F127 in 2 months. The electrophysiology examinations showed significant neuroretinal toxicity of Matrigel and Pluronic F127 but good biocompatibility of ECE. The neuroretinal toxicity of Matrigel and Pluronic F127 and superior biocompatibility of ECE hydrogel suggests ECE as more appropriate biomaterial for use in research and potentially in intraocular application.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biocompatible Materials / administration & dosage
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Biocompatible Materials / chemistry
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Biocompatible Materials / toxicity*
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Collagen / administration & dosage
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Collagen / chemistry
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Collagen / toxicity*
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Drug Carriers / administration & dosage
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Drug Carriers / chemistry
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Drug Carriers / toxicity*
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Drug Combinations
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Female
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Hydrogels / administration & dosage
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Hydrogels / chemistry
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Hydrogels / toxicity*
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Laminin / administration & dosage
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Laminin / chemistry
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Laminin / toxicity*
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Poloxamer / administration & dosage
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Poloxamer / chemistry
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Poloxamer / toxicity*
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Polyamines / administration & dosage
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Polyamines / chemistry
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Polyamines / toxicity*
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Polyesters / administration & dosage
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Polyesters / chemistry
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Polyesters / toxicity*
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Proteoglycans / administration & dosage
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Proteoglycans / chemistry
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Proteoglycans / toxicity*
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Rabbits
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Retina / drug effects
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Retina / pathology
Substances
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Biocompatible Materials
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Drug Carriers
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Drug Combinations
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Hydrogels
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Laminin
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Polyamines
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Polyesters
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Proteoglycans
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poly(2-ethyl-2-oxazoline)
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Poloxamer
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matrigel
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polycaprolactone
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Collagen
Grants and funding
This work was supported by the funding from the National Science Council of the Republic of China, Taiwan (NSC 98-2622-E-033-011-CC1, NSC 99-2622-E-033-005-CC1 and 101-2320-B-182-027-MY3), and the Chang Gung Memorial Hospital funds (CMRPG 390311, CMRPG 380181, CMRPD180403 and BMRP440). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.