Endogenous n-3 polyunsaturated fatty acids (PUFAs) mitigate ovariectomy-induced bone loss by attenuating bone marrow adipogenesis in FAT1 transgenic mice

Drug Des Devel Ther. 2013 Jun 28:7:545-52. doi: 10.2147/DDDT.S45263. Print 2013.

Abstract

Aim: To investigate the effect of endogenous n-3 polyunsaturated fatty acids (PUFAs) on bone marrow adipogenesis under osteoporosis conditions.

Methods: A mouse osteoporosis model overexpressing the FAT1 gene from Caenorhabditis elegans and converting n-6 PUFAs to n-3 PUFAs endogenously was used.

Results: The mice presented significantly lower bone marrow adiposity (adipocyte volume/tissue volume, mean adipocyte number) but increased the bone parameters (bone mineral density, bone mineral content, bone volume/total volume) in the distal femoral metaphysis.

Conclusion: Endogenous n-3 PUFAs protect bone marrow adipogenesis, which provides a novel drug target.

Keywords: adipogenesis; antiosteoporosis; bone marrow; n-3 PUFAs.

MeSH terms

  • Adipogenesis*
  • Adiposity
  • Animals
  • Bone Marrow / metabolism*
  • Cadherins / genetics
  • Cadherins / physiology*
  • Core Binding Factor Alpha 1 Subunit / analysis
  • Fatty Acids, Omega-3 / physiology*
  • Female
  • Immunohistochemistry
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Osteoporosis / prevention & control*
  • Ovariectomy*
  • PPAR gamma / analysis

Substances

  • Cadherins
  • Core Binding Factor Alpha 1 Subunit
  • Fatty Acids, Omega-3
  • PPAR gamma
  • Runx2 protein, mouse
  • fat1 protein, mouse