A new diamond biosensor with integrated graphitic microchannels for detecting quantal exocytic events from chromaffin cells

Federico Picollo; Sara Gosso; Ettore Vittone; Alberto Pasquarelli; Emilio Carbone; Paolo Olivero; Valentina Carabelli

doi:10.1002/adma.201300710

A new diamond biosensor with integrated graphitic microchannels for detecting quantal exocytic events from chromaffin cells

Adv Mater. 2013 Sep 14;25(34):4696-700. doi: 10.1002/adma.201300710. Epub 2013 Jul 12.

Authors

Federico Picollo¹, Sara Gosso, Ettore Vittone, Alberto Pasquarelli, Emilio Carbone, Paolo Olivero, Valentina Carabelli

Affiliation

¹ Department of Physics, NIS Centre of Excellence, CNISM Research Unit - University of Torino, INFN Sez. Torino, via P. Giuria 1, Torino, 10125, Italy. [email protected].

PMID: 23847004
DOI: 10.1002/adma.201300710

Abstract

An MeV ion-microbeam lithographic technique can be successfully employed for the fabrication of an all-carbon miniaturized cellular biosensor based on graphitic microchannels embedded in a single-crystal diamond matrix. The device is functionally characterized for the in vitro recording of quantal exocytic events from single chromaffin cells, with high sensitivity and signal-to-noise ratio, opening promising perspectives for the realization of monolithic all-carbon cellular biosensors.

Keywords: cellular biosensors; exocytosis; ion beam lithography; single-crystal diamond.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adrenal Glands / cytology
Animals
Biosensing Techniques*
Cells, Cultured
Chromaffin Cells / cytology*
Chromaffin Cells / metabolism
Diamond / chemistry*
Electrochemical Techniques
Exocytosis / physiology*
Male
Mice
Mice, Inbred C57BL
Microelectrodes
Miniaturization
Signal-To-Noise Ratio

Substances

Diamond