Emergence of multiple clade 2.3.2.1 influenza A (H5N1) virus subgroups in Vietnam and detection of novel reassortants

Virology. 2013 Sep;444(1-2):12-20. doi: 10.1016/j.virol.2013.06.005. Epub 2013 Jul 10.

Abstract

Phylogenetic analyses of 169 influenza A(H5N1) virus genomes were conducted for samples collected through active surveillance and outbreak responses in Vietnam between September 2010 and September 2012. While clade 1.1 viruses persisted in southern regions, three genetically distinct subgroups of clade 2.3.2.1 were found in northern and central Vietnam. The identification of each subgroup corresponded with detection of novel reassortants, likely due to their overlapping circulation throughout the country. While the previously identified clade 1.1 and A/Hubei/1/2010-like 2.3.2.1 genotypes remained the predominant viruses detected, four viruses were found to be reassortants between A/Hubei/1/2010-like (HA, NA, PB2, PB1, PA, NP) and A/duck/Vietnam/NCVD-885/2010-like (M, NS) viruses and one virus was identified as having A/duck/Vietnam/NCVD-885/2010-like HA, NA, PB1, and NP with A/Hubei/1/2010-like PB2 and PA genes. Additionally, clade 2.3.2.1 A/Hong Kong/6841/2010-like viruses, first detected in mid-2012, were identified as reassortants comprised of A/Hubei/1/2010-like PB2 and PA and A/duck/Vietnam/NCVD-885/2010-like PB1, NP, NA, M, NS genes.

Keywords: Evolution; Geographic distribution; H5N1; Highly pathogenic avian influenza virus; Orthomyxovirus; Phylogenetics; Reassortment.

MeSH terms

  • Animals
  • Cluster Analysis
  • Genotype
  • Influenza A Virus, H5N1 Subtype / classification*
  • Influenza A Virus, H5N1 Subtype / genetics*
  • Influenza A Virus, H5N1 Subtype / isolation & purification
  • Influenza in Birds / virology*
  • Molecular Sequence Data
  • Phylogeography*
  • Poultry
  • RNA, Viral / genetics*
  • Real-Time Polymerase Chain Reaction
  • Reassortant Viruses / classification*
  • Reassortant Viruses / genetics*
  • Reassortant Viruses / isolation & purification
  • Sequence Analysis, DNA
  • Vietnam

Substances

  • RNA, Viral