Pontibacter toksunensis sp. nov., isolated from soil, and emended descriptions of Pontibacter roseus and Pontibacter akesuensis

Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4462-4468. doi: 10.1099/ijs.0.053165-0. Epub 2013 Jul 12.

Abstract

A Gram-reaction-negative, rod-shaped, non-motile, red-pigmented bacterial strain, designated ZLD-7(T), was isolated from a soil sample collected from an arid area in Xinjiang Province in north-west China, and characterized by using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis showed that strain ZLD-7(T) was a member of the genus Pontibacter in the family Cytophagaceae, with sequence similarities of 93.7-96.2 % to type strains of other Pontibacter species. The only isoprenoid quinone of strain ZLD-7(T) was MK-7, and its major cellular fatty acids were summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I), iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The polar lipid profile consisted of phosphatidylethanolamine, one unidentified aminolipid and four unidentified polar lipids. The DNA G+C content was 47.8 mol%. On the basis of the evidence presented, it is proposed that strain ZLD-7(T) represents a novel species of the genus Pontibacter, for which the name Pontibacter toksunensis sp. nov. is proposed. The type strain is ZLD-7(T) ( = CCTCC AB 208003(T) = KCTC 23984(T)). Emended descriptions of Pontibacter roseus and Pontibacter akesuensis are also proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • China
  • Cytophagaceae / classification*
  • Cytophagaceae / genetics
  • Cytophagaceae / isolation & purification
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Molecular Sequence Data
  • Phosphatidylethanolamines / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phosphatidylethanolamines
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • phosphatidylethanolamine
  • menaquinone 7

Associated data

  • GENBANK/KC894746