Serum methylation levels of TAC1. SEPT9 and EYA4 as diagnostic markers for early colorectal cancers: a pilot study

Biomarkers. 2013 Aug;18(5):399-405. doi: 10.3109/1354750X.2013.798745.

Abstract

Objective: To identify methylated genes in serum with diagnostic potentials for early colorectal cancer (CRC).

Methods: Serum methylation levels of up to 12 genes were measured in two sets of serum samples with the second set from 26 stage I CRC patients and 26 age/gender-matched controls.

Results: Serum methylation levels of TAC1, SEPT9, and EYA4 were significant discriminants between stage I CRC and healthy controls. Combination of TAC1 and SEPT9 rendered 73.1% sensitivity with 92.3% specificity.

Conclusion: Serum methylation levels of TAC1. SEPT9 and EYA4 may be useful biomarkers for early detection of CRC though a validation study is necessary.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Area Under Curve
  • Biomarkers, Tumor / blood*
  • Biomarkers, Tumor / genetics
  • Case-Control Studies
  • Colorectal Neoplasms / blood*
  • Colorectal Neoplasms / diagnosis
  • Colorectal Neoplasms / genetics
  • DNA Methylation
  • Early Detection of Cancer
  • Female
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Pilot Projects
  • Prospective Studies
  • ROC Curve
  • Septins / blood*
  • Septins / genetics
  • Trans-Activators / blood*
  • Trans-Activators / genetics
  • Transmembrane Activator and CAML Interactor Protein / blood*
  • Transmembrane Activator and CAML Interactor Protein / genetics

Substances

  • Biomarkers, Tumor
  • EYA4 protein, human
  • TNFRSF13B protein, human
  • Trans-Activators
  • Transmembrane Activator and CAML Interactor Protein
  • SEPTIN9 protein, human
  • Septins