Carbon nanotube-based aptasensors for the rapid and ultrasensitive detection of bacteria

Methods. 2013 Oct;63(3):233-8. doi: 10.1016/j.ymeth.2013.07.008. Epub 2013 Jul 17.

Abstract

In this paper we present a new generation of potentiometric biosensors based on carbon nanotubes (transducer layer of the biosensor) and aptamers (sensing layer of the biosensor) for the ultralow and selective detection of microorganisms. We show that with these aptasensors we were able to detect a few CFU of the target bacteria almost in real-time, both in buffered and in real samples.

Keywords: Aptamers; Aptasensors; Bacteria; Carbon nanotubes; Potentiometry.

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Bacteria / isolation & purification*
  • Biosensing Techniques / methods
  • Electrodes
  • Nanotubes, Carbon / chemistry*
  • Potentiometry

Substances

  • Aptamers, Nucleotide
  • Nanotubes, Carbon