Abstract
Cell extracts of uric acid-grown Clostridium acidurici catalyzed the coupled reduction of NAD(+) and ferredoxin with formate at a specific activity of 1.3 U/mg. The enzyme complex catalyzing the electron-bifurcating reaction was purified 130-fold and found to be composed of four subunits encoded by the gene cluster hylCBA-fdhF2.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Clostridium / enzymology*
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Ferredoxins / metabolism*
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Formate Dehydrogenases / isolation & purification*
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Formate Dehydrogenases / metabolism*
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Formates / metabolism*
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Multigene Family
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NAD / metabolism*
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Protein Multimerization
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Protein Subunits / isolation & purification
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Protein Subunits / metabolism
Substances
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Ferredoxins
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Formates
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Protein Subunits
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NAD
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formic acid
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Formate Dehydrogenases